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KMID : 1143520160040020076
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Journal of Korean Society of Oral Health Science
2016 Volume.4 No. 2 p.76 ~ p.83
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Growth Susceptibility of Tetrodotoxin (TTX), Pufferfish, Against Streptococcus mutans and Staphylococcus aureus
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Kim Hyun-Dae
Kim Se-Yeon
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Abstract
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The objective of this study is to search the possibility of using the liver of pufferfish, a marine creature as natural antimicrobial by experimenting flesh sensitivity of tetrodotoxin extracted from the liver of pufferfish, a marine creature to Streptococcus mutans and Staphylococcus aureus . The tetrodonic acid (TDA), namely, inducer of tetrodotoxin was confirmed with High Performance Liquid Chromatography (HPLC) to detect and extract “tetrodotoxins fraction” of pufferfish. In order to measure the effect of tetrodotoxin on the growth of Streptococcus mutans and Staphylococcus aureus , it is added to each culture at each concentration of 0-10 nM. After they are measured with spectrophotometer, it is found that experimental group shows the power to inhibit the growth of Streptococcus mutans in 30 hours after cultivation in comparison with control group. Then, the growth curve of Staphylococcus aureus is in the similar type to that of S. mutans . It is found that the growth of S. aureus is inhibited in 24 hours after cultivation in the experimental group where 0.01?0.07 nM tetrodotoxin is contained. Further, the experimental group where 0.15-10 nM tetrodotoxin is contained mostly shows the power to inhibit strain in 4 hours after cultivation, more apparent than the growth inhibiting power of S. mutans . When measuring pH after cultivating experimental group in Streptococcus mutans culture for 24 hours by adding tetrodotoxin of each concentration, we found pH 7.4-7.7 by 4 hours before cultivation. The pH did not vary apparently in comparison to control group. When the contents of tetrodotoxin in each concentration increase in the S. aureus culture, the change in pH is not large in comparison to cultivation time. However, it rapidly decreased in experimental group in just 6 hours after cultivation. Thus, it is judged that it works to hinder the strain growth. As a result, tetrodotoxin is judged to inhibit the growth of Streptococcus mutans and Staphylococcus aureus.
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KEYWORD
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Growth susceptibility, Tetrodotoxin, Streptococcus mutans, Streptococcus aureus
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